RESEARCH TOPIC :
We are mainly interested in the molecular mechanisms of synaptic transmission and neuronal excitability. Our research focuses on 3 projects:
1- Molecular mechanisms underlying LGI1 (Leucine-rich Glioma-Inactivated 1) mediated fine tuning of neuronal excitability
2- Activity and Receptors of Botulinum Neurotoxins
3- Synaptic transmission modulation by subunits of the vesicular V-ATPase
1- Molecular mechanisms underlying LGI1 (Leucine-rich Glioma-Inactivated 1) fine tuning of neuronal excitability
LGI1 (leucine-rich, glioma-inactivated 1) is a soluble glycoprotein involved in two neurological affections: Autosomal Dominant Lateral Temporal Epilepsy (ADLTE) and certain autoimmune forms of Limbic Encephalitis (LE). In LGI1 dependent LE patients, autoimmunity is characterized by the presence of anti-LGI1 autoantibodies. In these affection, epilepsy is associated with memory loss and psychiatric disorders.
LGI1 interacts with the inactive metalloproteases ADAM22 and 23 and has been shown to be present in multi-molecular synaptic complexes including Kv1 channels and AMPA receptors. For several years, our team has been performing diagnostic tests for LGI1-dependent autoimmune LE using immunoprecipitation of Kv1 channels radio-labelled with iodinated Dendrotoxin.
Mutations in the gene coding for LGI1 mainly cause haplo-insufficiency mainly due to lack of protein secretion. Epilepsy accompanied by auditory aura characterize the majority of patients. The molecular mechanisms by which LGI1 haplo-insufficiency leads to neuronal excitability increase are poorly understood. Our data (Seagar et al., 2017) show that the neuronal excitability increase is due to a decrease in the expression of axonal Kv1 channels that play an important role in the regulation of crucial neuronal properties such as excitability, axonal conduction, and synaptic strength. 
2- Activity and Receptors of Botulinum Neurotoxins Botulinum neurotoxins secreted by bacteria are responsible for human and animal botulism. It results in peripheral dysautonomia and marked potentially lethal flaccid paralysis. Moreover, these neurotoxins are of major therapeutic interest for the treatment of multiple neurological conditions. Using laboratory-specific biological tools and a method based on Surface Plasmon Resonance, we have developed over the past ten years, rapid and ultra-efficient assays to specifically quantify the proteolytic activity of several serotypes of botulinum neurotoxins in patients sera. In collaboration with IPSEN, these tests enabled us to validate the activity of pharmaceutical batches. 
In collaboration with CILOA (Montpellier), we have recently developed an exosomal system allowing the expression, in a native conformation, at the surface of recombinant exosomes different membrane receptors with one or more transmembrane domains (Desplantes et al., 2017). We validated the usefulness of this methodology for the study of G-protein coupled receptors pharmacology and botulinum neurotoxin receptors. Our current approach aims at deciphering, through cellular biology, biochemistry and modeling approaches, the molecular mechanisms that govern the recognition of botulinum neurotoxins by their neuronal receptors. We recently uncovered a directed inyteraction between the extracellular domain of synaptotagmin with gangliosides (Flores et al. 2019). We suggest that this complex represent the neuronal membrane receptor of botulinum neurotoxin type B.  3- Synaptic transmission modulation by subunits of the vesicular V-ATPase
V-ATPase (vacuolar proton ATPase) is a multimolecular nano-motor found in all eukaryotic cells. It is composed of reversibly associated two multimolecular sectors: an extra-membranous sector V1 that hydrolyzes ATP and a transmembrane sector V0 that transports protons. The primary function of this nano-motor is the active (ATP-dependent) transport of protons. It acidifies intracellular compartments and plays an important role in many physiological processes. The coupling between ATP hydrolysis and proton transport by the V-ATPase plays a crucial role in the acidification of synaptic vesicles and thus their loading in neurotransmitter molecules. V0-sector subunits of the V-ATPase interact with SNARE proteins and independently of proton transport, a role of these subunits in modulating SNARE-dependent neurotransmitter release has emerged in recent years. We are particularly interested in the implication of the subunits "V0c" and "V0d" in the modulation of neurotransmitter release.

PROJECTS :
1- Molecular mechanisms underlying LGI1 (Leucine-rich Glioma-Inactivated 1) mediated fine tuning of neuronal excitability We are interested in the effects of LGI1 and autoantibodies on the regulation of Kv1 channel expression and its consequences on neuronal excitability and synaptic transmission.
2-Activity and Receptors of Botulinum Neurotoxins
Our current approach aims at deciphering, through cellular biology, biochemistry and modeling approaches, the molecular mechanisms that govern the recognition of botulinum neurotoxins by their neuronal receptors.
3- Synaptic transmission modulation by subunits of the vesicular V-ATPase
We are particularly interested in the implication of the subunits "V0c" and "V0d" in the modulation of neurotransmitter release.
FUNDING :
ANR LoGik
FORMER MEMBERS :
AMENDOLA Julien, BRECHET Aline, CHAHINIAN Henri, DE SAN FELICIANO Marina , DESPLANTES Richard, DRIHEM Chiraz, ERARD-GARCIA Madeleine, FLORES Alessandra, GOUGOT Julie, LEVY Annie , MAULET Yves , MOUTOT Nicole , RAMIREZ FRANCO José Jorge, SAMARI Nada, VAN RENTERGHEM Catherine, YOUSSOUF Fahamoe .
PUBLICATIONS :
- 2023 - Azzaz F, Hilaire D, Fantini J.
Structural basis of botulinum neurotoxin serotype A1 binding to human SV2A or SV2C receptors. Chem Biol Interact, 373 : 110384 - 2023 - Azzaz F, Chahinian H, Yahi N, Fantini J, Di Scala C.
AmyP53 Prevents the Formation of Neurotoxic β-Amyloid Oligomers through an Unprecedent Mechanism of Interaction with Gangliosides: Insights for Alzheimer's Disease Therapy. Int J Mol Sci, 24 : 1760 - 2022 - Azzaz F, Yahi N, Di Scala C, Chahinian H, Fantini J.
Ganglioside binding domains in proteins: Physiological and pathological mechanisms Advances in Protein Chemistry and Structural Biology, 128 : 289-324 - 2022 - Azzaz F, Fantini J.
The epigenetic dimension of protein structure Biomolecular Concepts, 13 : 55-60 - 2022 - Ramirez-Franco JJ, Debreux K, Extrémet J, Maulet Y, Belghazi M, Villard C, Sangiardi M, Youssouf F, El Far L, Lévêque C, Debarnot C, Marchot P, , Debanne D, Russier M, Seagar M, Irani S, El Far O.
Patient-derived antibodies reveal the subcellular distribution and heterogeneous interactome of LGI1 Brain, doi: 10.1093/brain/awac218 - 2022 - Extrémet J, El Far O, Ankri N, Irani SR, Debanne D, Russier M.
An Epitope-Specific LGI1-Autoantibody Enhances Neuronal Excitability by Modulating Kv1.1 Channel Cells, 11 : 2713 - 2022 - Ramirez-Franco JJ, Azzaz F, Sangiardi M, Ferracci G, Youssouf F, Popooff MR, Seagar M, Lévêque C, Fantini J, El Far O.
Molecular landscape of BoNT/B bound to a membrane-inserted synaptotagmin/ganglioside complex Cell Mol Life Sci, 79 : 496 - 2022 - Azzaz F, Hilaire D, Fantini J.
Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft Biomolecule, 12 : 1821 - 2022 - Di Scala C, Amstrong N, Chahinian H, Chabrière E, Fantini J, Yahi N.
AmyP53, a Therapeutic Peptide Candidate for the Treatment of Alzheimer's and Parkinson's Disease: Safety, Stability, Pharmacokinetics Parameters and Nose-to Brain Delivery Int J Mol Sci, 23 : 13383 - 2021 - Fantini J, Chahinian H, Yahi N.
Leveraging coronavirus binding to gangliosides for innovative vaccine and therapeutic strategies against COVID-19 Biochemical and Biophysical Research Communication, 538 : 132-136 - 2021 - Fantini J, Yahi N, Azzaz F, Chahinian H.
Structural dynamics of SARS-CoV-2 variants: A health monitoring strategy for anticipating Covid-19 outbreaks Journal of Infection, 83 : 197-206 - 2021 - Yahi N, Di Scala C, Chahinian H, Fantini J.
Innovative treatment targeting gangliosides aimed at blocking the formation of neurotoxic α-synuclein oligomers in Parkinson's disease Glycoconjugate Journal - 2021 - Ramirez-Franco JJ, Azzaz F, Sangiardi M, Ferracci Géraldine, Youssouf F, Popoff Michel Robert, Seagar M, Lévêque C, Fantini J, El Far O.
The protein-binding pocket of Botulinum neurotoxin B accommodates a preassembled synaptotagmin / ganglioside complex BioRxiv - 2020 - Fantini J, Chahinian H, Yahi N.
Synergistic antiviral effect of hydroxychloroquine and azithromycin in combination against SARS-CoV-2: What molecular dynamics studies of virus-host interactions reveal International Journal Antimicrobial Agents, 56 : 106020 - 2020 - Fantini J, Chahinian H, Yahi N.
Progress toward Alzheimer's disease treatment: Leveraging the Achilles' heel of Aβ oligomers? Protein Science, 29 : 1748-1759 - 2020 - Fantini J, Chahinian H, Yahi N.
Leveraging coronavirus binding to gangliosides for innovative vaccine and therapeutic strategies against COVID-19 Biochemical and Biophysical Research Communications, doi: 10.1016/j.bbrc.2020.10.015. - 2020 - Ramberger M, Berretta A,Tan JMM, Sun B, Michael S, Yeo T, Theorell J, Bashford-Rogers R, Paneva S, O'Dowd V, Dedi N,, Topia S, Griffin R, Ramirez-Franco JJ, El Far O, Baulac S, Leite M, Sen A, Jeans A, McMillan D, Marshall D, Antony D, Lightwood D, Waters P, Irani SR.
Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms Brain, 143 : 1731-1745 - 2019 - Rama S, Boumedine N, Sangiardi M, Youssouf F, Maulet Y, Lévêque C, Belghazi M, Seagar M, Debanne D, El Far O.
Chromophore-assisted light inactivation of the V-ATPase V0c subunit inhibits neurotransmitter release downstream of synaptic vesicle acidification Molecular Neurobiology, 56 : 3591-3602 - 2019 - Flores A, Ramirez-Franco JJ, Desplantes R, Debreux K, Ferracci G, Wernert F, Blanchard MP, Maulet Y, Youssouf F, Sangiardi M, Iborra C, Popoff MR, Seagar M, Fantini J, Lévêque C, , El Far O.
Gangliosides interact with synaptotagmin to form the high-affinity receptor complex for botulinum neurotoxin B PNAS, www.pnas.org/cgi/doi/10.1073/pnas.1908051116 - 2018 - Coralie Di Scala, Fantini J, Yahi N, Francisco J. Barrantes, Chahinian H.
Anandamide Revisited: How Cholesterol and Ceramides Control Receptor-Dependent and Receptor-Independent Signal Transmission Pathways of a Lipid Neurotransmitter Biomolecules, 8 - 2018 - Debanne D, El Far O.
Pre- and post-synaptic effects of LGI1 autoantibodies in a murine model of limbic encephalitis Brain, 141 : 3092-3095 - 2018 - Huang Y, Huang S, Di Scala C, Wang Q, Wandall HH, Fantini J, Zhang YQ.
The glycosphingolipid MacCer promotes synaptic bouton2 formation in Drosophila by interacting with Wnt eLife, 7 : e38183 - 2017 - Desplantes R, Lévêque C, Muller B, Loterzio M, Ferracci G, Popoff M, Seagar M, Mamoun R, El Far O.
Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor Scientific Reports, 7 : 1032 - 2017 - Seagar M, Russier M, Caillard O, Maulet Y, Fronzaroli-Molinieres L, De San Feliciano M, Boumedine N, Rodriguez L, Zbili M, Usseglio F, Formisano-Treziny C, Youssouf F, Sangiardi M, Boillot M, Baulac S, Benitez MJ, Garrido JJ, Debanne D, , El Far O.
LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels Proceedings of the National Academy of Sciences USA, 114 : 7719-7724 - 2017 - Connan C, Voillequin M, Chavez CV, Mazuet C1,, Lévêque C, Vitry S, Vandewalle A, Popoff MR.
Botulinum neurotoxin type B uses a distinct entry pathway mediated by CDC42 into intestinal cells versus neuronal cells. Cellular Microbiology, 19 : e12738 - 2015 - Lévêque C, Ferracci G, Maulet Y, Grand-Masson C, Seagar M, El Far O.
A chip-based assay for botulinum neurotoxin A activity in pharmaceutical preparations. Appl Microbiol Biotechnol. , 99 : 4355-4360 - 2015 - Amendola J, Boumedine N, Sangiardi M, El Far O.
Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording Scientific Reports, 5 : 14455 - 2015 - Lévêque C, Ferracci G, Maulet Y, Mazuet C, Popoff MR, Blanchard MP, Seagar M, El Far O.
An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E Scientific Reports, 5 : 17953 - 2015 - Hammond C, El Far O, Seagar M.
Neurotransmitter release Cellular and molecular neurophysiology - 2015 - El Far O, Seagar M.
The synaptic vesicle V-ATPase: a regulatory link between loading and fusion? Presynaptic terminals - 2014 - Lévêque C, Ferracci G, Maulet Y, Mazuet C, Popoff M, Seagar M, El Far O.
Direct biosensor detection of botulinum neurotoxin endopeptidase activity in sera from patients with type A botulism Biosensors and Bioelectronics, 57 : 207-212 - 2014 - Mlayah-Bellalouna S, Dufour M, Mabrouk S, Mejdoub, H, Carlier E, Othman H, Belghazi M, Tarbe M, Goaillard JM, Gigmes D, Seagar M, El Ayeb M, Debanne D, Srairi-Abid N.
AaTX1, from Androctonus australis scorpion venom: Purification, synthesis and characterization in dopaminergic neurons Toxicon, 92 : 14-23 - 2013 - Lévêque C, Ferracci G, Maulet Y, Grand-Masson C, Blanchard MP, Seagar M, El Far O.
A substrate sensor chip to assay the enzymatic activity of Botulinum neurotoxin A. Biosensors & Bioelectronics, 49 : 276-281 - 2012 - Slezak M, Grosche A, Niemiec A,Tanimoto N, Pannicke T, Münch TA , Crocker B, Isope P, Härtig W, Beck SC, Huber G, Ferracci G, Perraut M, Rebert M, Miehe M, Demais V, Lévêque C, Metzger D, Szklarczyk K, Przewlocki R, Seeliger MW, Sage-Ciocca D, Hirrlinger J, Reichenbach A, Reibel S, Prrieger FW.
Relevance of exocytotic glutamate release from retinal glia. Neuron, 74 : 504-516 - 2012 - Belzeaux R, Bergon A, Jeanjean V, Loriod B, , Formisano-Treziny C, Verrier L, Loundou A, Baumstarck-Barrau K, Boyer L, Gall V, , Gabert J, Nguyen C, Azorin JM, Naudin J, Ibrahim EC.
Responder and nonresponder patients exhibit different peripheral transcriptional signatures during major depressive episodes Translational Psychiatry, 2 : e185 - 2011 - El Far O, Seagar M.
A role for V-ATPase subunits in synaptic vesicle fusion? Journal of Neurochemistry, 117 : 603-612 - 2011 - El Far O, Seagar M.
SNARE, V-ATPase and neurotransmission Med Sci (Paris), 27 : 28-31 - 2011 - Ferracci G, Marconi S, Mazuet C, Jover E, Blanchard MP, Seagar M, Popoff M, Lévêque C.
A label-free biosensor assay for botulinum neurotoxin B in food and human serum. Anal Biochem, 410 : 281-288 - 2010 - Di Giovanni J, Boudkkazi S, Mochida S, Bialowas A, Samari N, Lévêque C, Youssouf F, Brechet A, Iborra C, Maulet Y, Moutot N, Debanne D, Seagar M, El Far O.
V-ATPase membrane sector associates with synaptobrevin to modulate neurotransmitter release. Neuron, 67 : 268-279 - 2010 - Di Giovanni J, Iborra C, Maulet Y, Lévêque C, El Far O, Seagar M.
Calcium-dependent regulation of SNARE-mediated membrane fusion by calmodulin J Biol Chem, 285 : 23665-23675 - 2010 - Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE.
A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proc Natl Acad Sci USA, 107 : 3517-3521 - 2010 - ElBassiouni S, Amendola J, Durand J, Heckman C J.
Evidence from computer simulations for alterations in the membrane biophysical properties and dendritic processing of synaptic inputs in mutant SOD1 motoneurons J Neurosci, 30 : 5544-5558 - 2008 - Marconi S, Ferracci G, Berthomieu M, Kozaki S, Miquelis R, Boucraut J, Seagar M, Lévêque C.
A protein chip membrane-capture assay for botulinum neurotoxin activity. Toxicol Appl Pharmacol, 233 : 439-446 - 2008 - Moutot N, De Haro L, Seagar M.
Distinct evolution of calcium channel antibody types in Lambert-Eaton myasthenic syndrome. J Neuroimmunol, 197 : 47-53 - 2008 - Marqueze B, Moutot N, Sakkou-Norton M, Lévêque C, Ji Y, Cornet V, Hsiao WL, Seagar M.
Toxicity and endocytosis of spinocerebellar ataxia type 6 polyglutamine domains: role of myosin IIb. Traffic, 9 : 1088-1100 - 2007 - Bories C, Amendola J, Lamotte d'Incamps B, Durand J.
Early electrophysiological abnormalities in lumbar motoneurons in a transgenic mouse model of amyotrophic lateral sclerosis Eur J Neurosci, 25 : 451-459 - 2006 - Lacoste C, Barthaux V, Iborra C, Seagar M, Erard-Garcia M.
MAU-8 is a Phosducin-like Protein required for G protein signaling in C. elegans. Dev Biol, 294 : 181-191 - 2006 - Moutot N, Mansuelle P, Alcaraz G, Dos Santos RG, Cordeiro MN, De Lima ME, Seagar M, Van Renterghem C.
Phoneutria nigriventer toxin 1: a novel, state-dependent inhibitor of neuronal sodium channels that interacts with micro conotoxin binding sites. Mol Pharmacol, 69 : 1931-1937 - 2006 - Moutot N, De Haro L, Santos RG, Mori Y, Seagar M.
Phoneutria nigriventer omega-Phonetoxin IIA: a new tool for anti-calcium channel autoantibody assays in Lambert-Eaton myasthenic syndrome. Neurobiol Dis, 22 : 57-63 - 2006 - Durand J, Amendola J, Bories C, Lamotte d'Incamps B.
Early abnormalities in transgenic mouse models of amyotrophic lateral sclerosis J Physiol Paris, 99 : 211-220 - 2005 - Ferracci G, Miquelis R, Kozaki S, Seagar M, Lévêque C.
Synaptic vesicle chips to assay botulinum neurotoxins. Biochem J, 391 : 659-66 - 2004 - Ferracci G, Seagar M, Joël C, Miquelis R, Lévêque C.
Real time analysis of intact organelles using surface plasmon resonance. Anal Biochem, 334 : 367-375 - 2004 - Moutot N, De Haro L, Seagar M.
[Dosage and specificity of anti-calcium channel antibodies in Lambert-Eaton myasthenic syndrome]. Rev Neurol (Paris), 165 : S28-S34 - 2004 - De Haro L, Ferracci G, Opi S, Iborra C, Quetglas S, Miquelis R, Lévêque C, Seagar M.
Ca2+/calmodulin transfers the membrane-proximal lipid-binding domain of the v-SNARE synaptobrevin from cis to trans bilayers. Proc Natl Acad Sci U S A, 101 : 1578-1583 - 2004 - Amendola J, Verrier B, Roubertoux B, Durand J.
Altered sensorimotor development in a transgenic mouse model of amyotrophic lateral sclerosis Eur J Neurosci, 20 : 2822-2826 - 2003 - De Haro L, Quetglas S, Iborra C, Lévêque C, Seagar M.
Calmodulin-dependent regulation of a lipid binding domain in the v-SNARE synaptobrevin and its role in vesicular fusion. Biol Cell, 95 : 459-464 - 2002 - Quetglas S, Iborra C, Sasakawa N, De Haro l, Kumakura K, Sato K, Lévêque C, Seagar M.
Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosis. EMBO J, 21 : 3970-3979 - 2002 - Dos Santos RG, Van Renterghem C, Moutot N, Mansuelle P, Cordeiro MN, Diniz CR, Mori Y, De Lima ME, Seagar M.
Phoneutria nigriventer omega-phonetoxin IIA blocks the Cav2 family of calcium channels and interacts with omega-conotoxin-binding sites. J Biol Chem, 277 : 13856-13862 - 2001 - Seagar M, Quetglas S, Iborra C, Lévêque C.
Le complexe SNARE au coeur de la fusion membranaire Med. Sci, 5 : 669-674 - 2000 - Couraud F, Seagar M.
[Calcium channels and migraine]. Pathol Biol (Paris), 48 : 642-647 - 2000 - Van Renterghem C, Iborra C, Moutot N, Lelianova V, Ushkaryov Y, Seagar M.
alpha-latrotoxin forms calcium-permeable membrane pores via interactions with latrophilin or neurexin. Eur J Neurosci, 12 : 3953-3962 - 2000 - Quetglas S, Lévêque C, Miquelis R, Sato K, Seagar M.
Ca2+-dependent regulation of synaptic SNARE complex assembly via a calmodulin- and phospholipid-binding domain of synaptobrevin. Proc Natl Acad Sci U S A, 97 : 9695-9700 - 2000 - Marqueze B, Berton F, Seagar M.
Synaptotagmins in membrane traffic: which vesicles do the tagmins tag? Biochimie, 82 : 409-420 - 2000 - Berton F, Cornet V, Iborra C, Garrido J, Dargent B, Fukuda M, Seagar M, Marqueze B.
Synaptotagmin I and IV define distinct populations of neuronal transport vesicles. Eur J Neurosci, 12 : 1294-1302 - 2000 - Sampo B, Tricaud N, Lévêque C, Seagar M, Couraud F, Dargent B.
Direct interaction between synaptotagmin and the intracellular loop I-II of neuronal voltage-sensitive sodium channels. Proc Natl Acad Sci U S A, 97 : 3666-3671 - 2000 - Lévêque C, Boudier JA, Takahashi M, Seagar M.
Calcium-dependent dissociation of synaptotagmin from synaptic SNARE complexes. J Neurochem, 74 : 367-374 - 1999 - Boudier JA, Moutot N, Boudier JL, Iborra C, Takahashi M, Seagar M.
Redistribution of presynaptic proteins during alpha-latrotoxin-induced release of neurotransmitter and membrane retrieval at the frog neuromuscular junction. Eur J Neurosci, 11 : 3449-3456 - 1999 - Seagar M, Lévêque C, Charvin N, Marqueze B, Moutot N, Boudier JA, Boudier JL, Shoji-Kasai Y, Sato K.
Interactions between proteins implicated in exocytosis and voltage-gated calcium channels. Philos Trans R Soc Lond B Biol Sci, 354 : 289-297 - 1999 - Raymond C, Walker D, Bichet D, Iborra C, Moutot N, Seagar M, De Waard M.
Antibodies against the beta subunit of voltage-dependent calcium channels in Lambert-Eaton myasthenic syndrome. Neuroscience, 90 : 269-277 - 1998 - Seagar M, Takahashi M.
Interactions between presynaptic calcium channels and proteins implicated in synaptic vesicle trafficking and exocytosis. J Bioenerg Biomembr, 30 : 347-356 - 1998 - Lévêque C, Pupier S, Marqueze B, Geslin L, Kataoka M, Takahashi M, De Waard M, Seagar M.
Interaction of cysteine string proteins with the alpha1A subunit of the P/Q-type calcium channel. J Biol Chem, 273 : 13488-13492 - 1998 - Seagar M, Lévêque C, Charvin N, Marqueze B, Moutot N, Boudier JA, Boudier JL, Kataoka M, Shoji-Kaisai Y, Takahashi M.
Interactions between proteins implicated in exocytosis and voltage-gated calcium channels. Eur J Neurosci, 10 : 12501 - 1997 - Charvin N, Lévêque C, Walker D, Berton F, Raymond C, Kataoka M, Shoji-Kasai Y, Takahashi M, De Waard M, Seagar M.
Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel. EMO J, 16 : 4591-4596 - 1997 - Pupier S, Lévêque C, Marqueze B, Kataoka M, Takahashi M, Seagar M.
Cysteine string proteins associated with secretory granules of the rat neurohypophysis. J Neurosci, 17 : 2722-2727 - 1997 - Berton F, Iborra C, Boudier JA, Seagar M, Marqueze B.
Developmental regulation of synaptotagmin I, II, III, and IV mRNAs in the rat CNS. J Neurosci, 17 : 1206-1216 - 1996 - Arsac C, Raymond C, Moutot N, Dargent B, Couraud F, Poget J, Seagar M.
Immunoassays fail to detect antibodies against neuronal calcium channels in amyotrophic lateral sclerosis serum. Ann Neurol, 40 : 695-700 - 1996 - Moutot N, Charvin N, Lévêque C, Sato K, Nishiki T, Kozaki S, Takahashi M, Seagar M.
Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. J Biol Chem, 271 : 6567-6570 - 1996 - Boudier JA, Charvin N, Boudier JL, Fathallah M, Tagaya M, Takahashi M, Seagar M.
Distribution of components of the SNARE complex in relation to transmitter release sites at the frog neuromuscular junction. Eur J Neurosci, 8 : 545-552 - 1995 - El Far O, Marqueze B, Lévêque C, Moutot N, Lang B, Newsom-Davis J, Yoshida A, Takahashi M, Seagar M.
Antigens associated with N- and L-type calcium channels in Lambert-Eaton myasthenic syndrome. J Neurochem, 64 : 1696-1702 - 1995 - El Far O, Charvin N, Lévêque C, Moutot N, Takahashi M, Seagar M.
Interaction of a synaptobrevin (VAMP)-syntaxin complex with presynaptic calcium channels. FEBS Lett, 361 : 101-105 - 1995 - Kim JI, Takaashi M, Moutot N, Seagar M, Ohtake A, Sato K.
Tyr13 is essential for the binding of omega-conotoxin MVIIC to the P/Q-type calcium channel. Biochem Biophys Res Commun, 214 : 305-309 - 1995 - Marqueze B, Boudier JA, Mizuta M, Inagaki M, Seino S, Seagar M.
Cellular localization of synaptotagmin I, II, and III mRNAs in the central nervous system and pituitary and adrenal glands of the rat. J Neurosci, 15 : 4906-4917 - 1995 - Moutot N, Lévêque C, Sato K, Kato R, Takahashi M, Seagar M.
Properties of omega conotoxin MVIIC receptors associated with alpha 1A calcium channel subunits in rat brain. FEBS Lett, 366 : 21-25 - 1995 - Moutot N, Lang B, Newsom-Davis J, Seagar M.
Binding of Lambert-Eaton myasthenic syndrome IgG to synaptosomal proteins does not correlate with an inhibition of calcium uptake. Neurosci Lett, 187 : 11(-118 - 1995 - Seagar M, Moutot N, Lévêque C, Marqueze B, Pouget J.
Anti-calcium channel autoantibodies in amyotrophic lateral sclerosis. A review of the evidence. Adv Neurol, 68 : 67-72 - 1994 - Lévêque C, El Far O, Moutot N, Sato K, Kato R, Takahashi M, Seagar M.
Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. A complex implicated in synaptic vesicle exocytosis. J Biol Chem, 269 : 6306-6312 - 1993 - El Far O, Moutot N, Lévêque C, David P, Marqueze B, Lang B, Newom-Davis J, Hoshino T, Takahashi M, Seagar M.
Synaptotagmin associates with presynaptic calcium channels and is a Lambert-Eaton myasthenic syndrome antigen. Ann N Y Acad Sci, 707 : 382-385 - 1993 - David P, Moutot N, Lévêque C, El Far O, Takahashi M, Seagar M.
Interaction of synaptotagmin with voltage gated calcium channels: a role in Lambert-Eaton myasthenic syndrome? Neuromuscul Disord, 3 : 451-454 - 1993 - David P, El Far O, Moutot N, Poupon MF, Takahashi M, Seagar M.
Expression of synaptotagmin and syntaxin associated with N-type calcium channels in small cell lung cancer. FEBS Lett, 326 : 135-139 - 1993 - Seagar M, Moutot N, Lévêque C, El Far O, David P, Marqueze B, Lang B, Newsom-Davis J, Hoshino T, Takahashi M.
A Lambert-Eaton myasthenic syndrome antigen associated with presynaptic calcium channels. Biochem Soc Trans, 21 : 423-428 - 1993 - Moutot N, El Far O, Lévêque C, David P, Marqueze B, Lang B, Newsom-Davis J, Hoshino T, Takahashi M, Seagar M.
Synaptotagmin: a Lambert-Eaton myasthenic syndrome antigen that associates with presynaptic calcium channels. J Physiol Paris, 87 : 37-41 - 1993 - McEnery MW, Snowman AM, Seagar M, Copeland TD, Takahashi M.
Immunological characterization of proteins associated with the purified omega-conotoxin GVIA receptor. Ann N Y Acad Sci, 707 : 386-391 - 1993 - Grignon S, Seagar M, Couraud F.
Omega-conotoxin sensitive calcium channels in cerebellar granule cells are not coupled to [3H]glutamate release. Neurosci Lett, 155 : 8è-91 - 1992 - Lévêque C, Hoshino T, David P, Shoji-Kasai Y, Leys K, Omori A, Lang B , El Far O, Sato K, Newsom-Davis J, Takahashi M, Moutot N, Seagar M.
The synaptic vesicle protein synaptotagmin associates with calcium channels and is a putative Lambert-Eaton myasthenic syndrome antigen. Proc Natl Acad Sci U S A, 89 : 3625-3629 - 1992 - De Waard M, Seagar M, Feltz A, Couraud F.
Inositol phosphate regulation of voltage-dependent calcium channels in cerebellar granule neurons. Neuron, 9 : 497-503 - 1988 - Marqueze B, Moutot N, Lévêque C, Couraud F.
Characterization of the omega-conotoxin-binding molecule in rat brain synaptosomes and cultured neurons. Mol Pharmacol, 34 : 87-90
Equipe suivante Retour au sommaire |