ANNEE : 2000Binding of Ala-scanning analogs of omega-conotoxin MVIIC to N- and P/Q-type calcium channels. AUTEURS :
Sato K, Raymond C, Moutot N
, Sasaki T, Ohtake A, Minami K, Van Renterghem C, Kim JI, Takahashi M, Seagar M
FEBS LettN° Pubmed : 10713260
omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are essential for binding to both N- and P/Q-type channels, whereas Lys(4) and Arg(22) are important for binding to P/Q-type channels. These results suggest that MVIIC interacts with P/Q-type channels via a large surface, in good agreement with previous observations using chimeric analogs.