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ANNEE : 1980

Purification and chemical characterization of melittin and acetylated derivatives.

AUTEURS : Maulet Y, Mathey-Prevot B, Kaiser G, Rüegg UT, Fulpius BW.

REVUE : Biochim Biophys Acta
N° Pubmed : 7437462

Melittin, the main basic and hydrophobic peptide of bee venom, displays marked detergent-like properties. At high peptide concentration, and depending on salt and pH, it forms a tetramer. This is prevented by using urea. A purification procedure in presence of 4.0 M urea was developed to prepare melittin in its monomeric form, free of other venom constituents such as N alpha-formyl melittin, degradation products of peptides and phospholipase A2. NH2-residues on the melittin molecule were modified by reaction with acetic anhydride to alter the asymmetrical charge distribution supposed to confer detergent-like properties to the molecule. This gave rise to di- and mono acetyl derivatives which could be used, once isolated, to study further the melittin structure-activity relationship.