LEVEQUE Christian UMR UNIS 1072 unité de neurobiologie

ORGANISATION

AXE DEBANNE Dominique

AXE EL FAR Oussama

PLATEFORME BIOMARQUEURS DIAGNOSTIC

SERVICES COMMUNS

	LEVEQUE Christian Ingénieur de recherche -IR TELEPHONE : 04 91 69 88 48 EMAIL : christian.leveque@univ-amu.fr
PUBLICATIONS : 2022 - Ramirez-Franco JJ, Debreux K, Extrémet J, Maulet Y, Belghazi M, Villard C, Sangiardi M, Youssouf F, El Far L, Lévęque C, Debarnot C, Marchot P, , Debanne D, Russier M, Seagar M, Irani S, El Far O. Patient-derived antibodies reveal the subcellular distribution and heterogeneous interactome of LGI1 Brain, doi: 10.1093/brain/awac218 2022 - Ramirez-Franco JJ, Azzaz F, Sangiardi M, Ferracci G, Youssouf F, Popooff MR, Seagar M, Lévęque C, Fantini J El Far O. Molecular landscape of BoNT/B bound to a membrane-inserted synaptotagmin/ganglioside complex Cell Mol Life Sci, 79 : 496 2021 - Ramirez-Franco JJ, Azzaz F, Sangiardi M, Ferracci Géraldine, Youssouf F, Popoff Michel Robert, Seagar M, Lévęque C, Fantini J El Far O. The protein-binding pocket of Botulinum neurotoxin B accommodates a preassembled synaptotagmin / ganglioside complex BioRxiv 2019 - Rama S, Boumedine N, Sangiardi M, Youssouf F, Maulet Y, Lévęque C, Belghazi M, Seagar M, Debanne D El Far O. Chromophore-assisted light inactivation of the V-ATPase V0c subunit inhibits neurotransmitter release downstream of synaptic vesicle acidification Molecular Neurobiology, 56 : 3591-3602 2019 - Flores A, Ramirez-Franco JJ, Desplantes R, Debreux K, Ferracci G, Wernert F, Blanchard MP, Maulet Y, Youssouf F Sangiardi M, Iborra C, Popoff MR, Seagar M, Fantini J, Lévęque C El Far O. Gangliosides interact with synaptotagmin to form the high-affinity receptor complex for botulinum neurotoxin B PNAS, www.pnas.org/cgi/doi/10.1073/pnas.1908051116 2017 - Desplantes R, Lévęque C, Muller B, Loterzio M, Ferracci G, Popoff M, Seagar M, Mamoun R, El Far O. Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor Scientific Reports, 7 : 1032 2017 - Connan C, Voillequin M, Chavez CV, Mazuet C1,, Lévęque C, Vitry S, Vandewalle A, Popoff MR. Botulinum neurotoxin type B uses a distinct entry pathway mediated by CDC42 into intestinal cells versus neuronal cells. Cellular Microbiology, 19 : e12738 2015 - Lévęque C, Ferracci G, Maulet Y, Grand-Masson C, Seagar M, El Far O. A chip-based assay for botulinum neurotoxin A activity in pharmaceutical preparations. Appl Microbiol Biotechnol. , 99 : 4355-4360 2015 - Lévęque C, Ferracci G, Maulet Y, Mazuet C, Popoff MR, Blanchard MP, Seagar M, El Far O. An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E Scientific Reports, 5 : 17953 2015 - Lévęque C, Ferracci G, Maulet Y, Mazuet C, Popoff MR, Blanchard MP, Seagar M, El Far O. An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E. Scientific Reports, 5:17953 2014 - Lévęque C, Ferracci G, Maulet Y, Mazuet C, Popoff M, Seagar M, El Far O. Direct biosensor detection of botulinum neurotoxin endopeptidase activity in sera from patients with type A botulism Biosensors and Bioelectronics, 57 : 207-212 2014 - Tauzin AS, Sulzenbacher G, Lafond M, Desseaux V, Reca IB, Perrier J, Bellincampi D, Fourquet P, Lévęque CGiardina T. Functional characterization of a vacuolar invertase from Solanum lycopersicum: post-translational regulation by N-glycosylation and a proteinaceous inhibitor. Biochimie, 101 : 39-49 2013 - Lévęque C, Ferracci G, Maulet Y, Grand-Masson C, Blanchard MP, Seagar M, El Far O. A substrate sensor chip to assay the enzymatic activity of Botulinum neurotoxin A. Biosensors & Bioelectronics, 49 : 276-281 2012 - Slezak M, Grosche A, Niemiec A,Tanimoto N, Pannicke T, Münch TA , Crocker B, Isope P, Härtig W, Beck SC, Huber G, Ferracci G, Perraut M, Rebert M, Miehe M, Demais V, Lévęque C, Metzger D, Szklarczyk K, Przewlocki R, Seeliger MW, Sage-Ciocca D, Hirrlinger J, Reichenbach A, Reibel S, Prrieger FW. Relevance of exocytotic glutamate release from retinal glia. Neuron, 74 : 504-516 2012 - Mazarqui H, Lévęque C, Bartnick D, Fantini J, Gouget T, Melone MA, Funke SA, Willbold D, Perrone L. A synthetic amino acid substitution of Tyr10 in Aβ peptide sequence yields a dominant negative variant in amyloidogenesis. Aging Cell, 11 : 530-541 2012 - Slezak M, Grosche A, Niemiec A, Tanimoto N, Pannicke T, Münch TA, Crocker B, Isope P, Härtig W, Beck SC, Huber G, Ferracci G, Perraut M, Reber M, Miehe M,, Demais V, Lévęque C, Metzger D, ..., Pfrieger FW. Relevance of exocytotic glutamate release from retinal glia Neuron, 74 : 504-516 2011 - Ferracci G, Marconi S, Mazuet C, Jover E, Blanchard MP, Seagar M, Popoff M, Lévęque C. A label-free biosensor assay for botulinum neurotoxin B in food and human serum. Anal Biochem, 410 : 281-288 2011 - Labasque M, Devaux JJ, Lévęque C, Faivre-Sarrailh C. Fibronectin type III-like domains of neurofascin-186 protein mediate gliomedin binding and its clustering at the developing nodes of Ranvier. J Biol Chem, 286 : 42426-42434 2010 - Di Giovanni J, Boudkkazi S, Mochida S, Bialowas A, Samari N, Lévęque C, Youssouf F, Brechet A, Iborra C Maulet Y, Moutot N, Debanne D, Seagar M, El Far O. V-ATPase membrane sector associates with synaptobrevin to modulate neurotransmitter release. Neuron, 67 : 268-279 2010 - Di Giovanni J, Iborra C, Maulet Y, Lévęque C, El Far O, Seagar M. Calcium-dependent regulation of SNARE-mediated membrane fusion by calmodulin J Biol Chem, 285 : 23665-23675 2010 - Girardet C, Blanchard MP, Ferracci G, Lévęque C, Moreno M, François-Bellan AM, Becquet D, Bosler O. Daily changes in synaptic innervation of VIP neurons in the rat suprachiasmatic nucleus: contribution of glutamatergic afferents Eur J Neurosci, 21 : 359-370 2008 - Marconi S, Ferracci G, Berthomieu M, Kozaki S, Miquelis R, Boucraut J, Seagar M, Lévęque C. A protein chip membrane-capture assay for botulinum neurotoxin activity. Toxicol Appl Pharmacol, 233 : 439-446 2008 - Pereira S, Massacrier A, Roll P, Verine A, Etienne-Grimaldi MC, Poitelon Y, Robaglia-Sclupp A, Jamali S, Roeckel-Trevisiol N.Lévęque C. Nuclear localization of a novel human syntaxin 1B isoform. Gene, 423 : 160-171 2008 - Marqueze B, Moutot N, Sakkou-Norton M, Lévęque C, Ji Y, Cornet V, Hsiao WL, Seagar M. Toxicity and endocytosis of spinocerebellar ataxia type 6 polyglutamine domains: role of myosin IIb. Traffic, 9 : 1088-1100 2008 - Royer-Zemmour B, Ponsole-Lenfant M, Gara H, Roll P, Lévęque C, Massacrier A, Ferracci A, Cillario J, Robaglia-Sclupp A, Vincentelli R, Cau P, Szpetowski P. Epileptic and developmental disorders of the speech cortex: ligand/receptor interaction of wild-type and mutant SRPX2 with the plasminogen activator receptor uPAR. Hum Mol Genet, 17 : 3617-3630 2007 - Ji Y, Ferracci G, Warley A, Ward M, Leung KY, Samsuddin S, Lévęque C, Queen L, Reebye V, Pal P, Gkaliagkousi E, Seagar M, Ferro A. beta-Actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc Natl Acad Sci U S A, 104 : 8839-8844 2005 - Ferracci G, Miquelis R, Kozaki S, Seagar M, Lévęque C. Synaptic vesicle chips to assay botulinum neurotoxins. Biochem J, 391 : 659-66 2004 - Ferracci G, Seagar M, Joël C, Miquelis R, Lévęque C. Real time analysis of intact organelles using surface plasmon resonance. Anal Biochem, 334 : 367-375 2004 - De Haro L, Ferracci G, Opi S, Iborra C, Quetglas S, Miquelis R, Lévęque C, Seagar M. Ca2+/calmodulin transfers the membrane-proximal lipid-binding domain of the v-SNARE synaptobrevin from cis to trans bilayers. Proc Natl Acad Sci U S A, 101 : 1578-1583 2003 - De Haro L, Quetglas S, Iborra C, Lévęque C, Seagar M. Calmodulin-dependent regulation of a lipid binding domain in the v-SNARE synaptobrevin and its role in vesicular fusion. Biol Cell, 95 : 459-464 2002 - Quetglas S, Iborra C, Sasakawa N, De Haro l, Kumakura K, Sato K, Lévęque C, Seagar M. Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosis. EMBO J, 21 : 3970-3979 2002 - Geib S, Sandoz G, Mabrouk K, Matavel A, Marchot P, Hoshi T, Villaz M, Ronjat M, Miquelis R, Lévęque C, De Waard M. Use of a purified and functional recombinant calcium-channel beta4 subunit in surface-plasmon resonance studies. Biochem J, 364 : 285-292 2001 - Seagar M, Quetglas S, Iborra C, Lévęque C. Le complexe SNARE au coeur de la fusion membranaire Med. Sci, 5 : 669-674 2000 - Quetglas S, Lévęque C, Miquelis R, Sato K, Seagar M. Ca2+-dependent regulation of synaptic SNARE complex assembly via a calmodulin- and phospholipid-binding domain of synaptobrevin. Proc Natl Acad Sci U S A, 97 : 9695-9700 2000 - Sampo B, Tricaud N, Lévęque C, Seagar M, Couraud F, Dargent B. Direct interaction between synaptotagmin and the intracellular loop I-II of neuronal voltage-sensitive sodium channels. Proc Natl Acad Sci U S A, 97 : 3666-3671 2000 - Lévęque C, Boudier JA, Takahashi M, Seagar M. Calcium-dependent dissociation of synaptotagmin from synaptic SNARE complexes. J Neurochem, 74 : 367-374 1999 - Seagar M, Lévęque C, Charvin N, Marqueze B, Moutot N, Boudier JA, Boudier JL, Shoji-Kasai Y, Sato K. Interactions between proteins implicated in exocytosis and voltage-gated calcium channels. Philos Trans R Soc Lond B Biol Sci, 354 : 289-297 1998 - Lévęque C, Pupier S, Marqueze B, Geslin L, Kataoka M, Takahashi M, De Waard M, Seagar M. Interaction of cysteine string proteins with the alpha1A subunit of the P/Q-type calcium channel. J Biol Chem, 273 : 13488-13492 1998 - Seagar M, Lévęque C, Charvin N, Marqueze B, Moutot N, Boudier JA, Boudier JL, Kataoka M, Shoji-Kaisai Y, Takahashi M. Interactions between proteins implicated in exocytosis and voltage-gated calcium channels. Eur J Neurosci, 10 : 12501 1998 - Seagar M, Moutot N, Raymond C, Charvin N, Lévęque C, Walker D, De Waard M. Omega-conotoxins : diagnostic tools for an autoimmune disease of the neuromuscular juction Toxicon, 36 : 1730-1731 1997 - Charvin N, Lévęque C, Walker D, Berton F, Raymond C, Kataoka M, Shoji-Kasai Y, Takahashi M, De Waard M, Seagar M. Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel. EMO J, 16 : 4591-4596 1997 - Pupier S, Lévęque C, Marqueze B, Kataoka M, Takahashi M, Seagar M. Cysteine string proteins associated with secretory granules of the rat neurohypophysis. J Neurosci, 17 : 2722-2727 1996 - Moutot N, Charvin N, Lévęque C, Sato K, Nishiki T, Kozaki S, Takahashi M, Seagar M. Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. J Biol Chem, 271 : 6567-6570 1995 - El Far O, Marqueze B, Lévęque C, Moutot N, Lang B, Newsom-Davis J, Yoshida A, Takahashi M, Seagar M Antigens associated with N- and L-type calcium channels in Lambert-Eaton myasthenic syndrome. J Neurochem, 64 : 1696-1702 1995 - El Far O, Charvin N, Lévęque C, Moutot N, Takahashi M, Seagar M. Interaction of a synaptobrevin (VAMP)-syntaxin complex with presynaptic calcium channels. FEBS Lett, 361 : 101-105 1995 - Moutot N, Lévęque C, Sato K, Kato R, Takahashi M, Seagar M. Properties of omega conotoxin MVIIC receptors associated with alpha 1A calcium channel subunits in rat brain. FEBS Lett, 366 : 21-25 1995 - Seagar M, Moutot N, Lévęque C, Marqueze B, Pouget J. Anti-calcium channel autoantibodies in amyotrophic lateral sclerosis. A review of the evidence. Adv Neurol, 68 : 67-72 1994 - Lévęque C, El Far O, Moutot N, Sato K, Kato R, Takahashi M, Seagar M. Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. A complex implicated in synaptic vesicle exocytosis. J Biol Chem, 269 : 6306-6312 1993 - El Far O, Moutot N, Lévęque C, David P, Marqueze B, Lang B, Newom-Davis J, Hoshino T, Takahashi M, Seagar M. Synaptotagmin associates with presynaptic calcium channels and is a Lambert-Eaton myasthenic syndrome antigen. Ann N Y Acad Sci, 707 : 382-385 1993 - David P, Moutot N, Lévęque C, El Far O, Takahashi M, Seagar M. Interaction of synaptotagmin with voltage gated calcium channels: a role in Lambert-Eaton myasthenic syndrome? Neuromuscul Disord, 3 : 451-454 1993 - Seagar M, Moutot N, Lévęque C, El Far O, David P, Marqueze B, Lang B, Newsom-Davis J, Hoshino T, Takahashi M. A Lambert-Eaton myasthenic syndrome antigen associated with presynaptic calcium channels. Biochem Soc Trans, 21 : 423-428 1993 - Moutot N, El Far O, Lévęque C, David P, Marqueze B, Lang B, Newsom-Davis J, Hoshino T, Takahashi M, Seagar M. Synaptotagmin: a Lambert-Eaton myasthenic syndrome antigen that associates with presynaptic calcium channels. J Physiol Paris, 87 : 37-41 1993 - Lang B, Johnston I, Leys K, Elrington G, Marqueze B, Lévęque C, Moutot N, Seagar M, Hoshino T, Takahashi M, Sugimori M, Cherksey BD, Llinas R. Autoantibody specificities in Lambert-Eaton myasthenic syndrome. Ann N Y Acad Sci, 681 : 382-393 1992 - Lévęque C, Hoshino T, David P, Shoji-Kasai Y, Leys K, Omori A, Lang B , El Far O, Sato K, Newsom-Davis J, Takahashi M, Moutot N, Seagar M. The synaptic vesicle protein synaptotagmin associates with calcium channels and is a putative Lambert-Eaton myasthenic syndrome antigen. Proc Natl Acad Sci U S A, 89 : 3625-3629 1990 - Lévęque C, Marqueze B, Couraud F, Seagar M. Polypeptide components of the apamin receptor associated with a calcium activated potassium channel. FEBS Lett, 275 : 185-189 1988 - Marqueze B, Moutot N, Lévęque C, Couraud F. Characterization of the omega-conotoxin-binding molecule in rat brain synaptosomes and cultured neurons. Mol Pharmacol, 34 : 87-90 1988 - Marqueze B, Moutot N, Lévęque C, Couraud F. Characterization of the omega-conotoxin-binding molecule in rat brain synaptosomes and cultured neurons. Mol Pharmacol, 34 : 87-90