SEAGAR Michael UMR UNIS 1072 unité de neurobiologie

ORGANISATION

AXE DEBANNE Dominique

AXE EL FAR Oussama

PLATEFORME BIOMARQUEURS DIAGNOSTIC

SERVICES COMMUNS

	SEAGAR Michael Directeur de recherche -DR1-Emérite TELEPHONE : 04 91 69 89 29 EMAIL : michael.seagar@univ-amu.fr
PUBLICATIONS : 2022 - Ramirez-Franco JJ, Debreux K, Extrémet J, Maulet Y, Belghazi M, Villard C, Sangiardi M, Youssouf F, El Far L, Lévęque C, Debarnot C, Marchot P, , Debanne D, Russier M, Seagar M, Irani S, El Far O. Patient-derived antibodies reveal the subcellular distribution and heterogeneous interactome of LGI1 Brain, doi: 10.1093/brain/awac218 2022 - Ramirez-Franco JJ, Azzaz F, Sangiardi M, Ferracci G, Youssouf F, Popooff MR, Seagar M, Lévęque C, Fantini J El Far O. Molecular landscape of BoNT/B bound to a membrane-inserted synaptotagmin/ganglioside complex Cell Mol Life Sci, 79 : 496 2021 - Ramirez-Franco JJ, Azzaz F, Sangiardi M, Ferracci Géraldine, Youssouf F, Popoff Michel Robert, Seagar M, Lévęque C, Fantini J El Far O. The protein-binding pocket of Botulinum neurotoxin B accommodates a preassembled synaptotagmin / ganglioside complex BioRxiv 2019 - Rama S, Boumedine N, Sangiardi M, Youssouf F, Maulet Y, Lévęque C, Belghazi M, Seagar M, Debanne D El Far O. Chromophore-assisted light inactivation of the V-ATPase V0c subunit inhibits neurotransmitter release downstream of synaptic vesicle acidification Molecular Neurobiology, 56 : 3591-3602 2019 - Flores A, Ramirez-Franco JJ, Desplantes R, Debreux K, Ferracci G, Wernert F, Blanchard MP, Maulet Y, Youssouf F Sangiardi M, Iborra C, Popoff MR, Seagar M, Fantini J, Lévęque C El Far O. Gangliosides interact with synaptotagmin to form the high-affinity receptor complex for botulinum neurotoxin B PNAS, www.pnas.org/cgi/doi/10.1073/pnas.1908051116 2017 - Desplantes R, Lévęque C, Muller B, Loterzio M, Ferracci G, Popoff M, Seagar M, Mamoun R, El Far O. Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor Scientific Reports, 7 : 1032 2017 - Seagar M, Russier M, Caillard O, Maulet Y, Fronzaroli-Molinieres L, De San Feliciano M, Boumedine N, Rodriguez L, Zbili MUsseglio F, Formisano-Treziny C, Youssouf F, Sangiardi M, Boillot M, Baulac S, Benitez MJ, Garrido JJ, Debanne D El Far O. LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels Proceedings of the National Academy of Sciences USA, 114 : 7719-7724 2015 - Lévęque C, Ferracci G, Maulet Y, Grand-Masson C, Seagar M, El Far O. A chip-based assay for botulinum neurotoxin A activity in pharmaceutical preparations. Appl Microbiol Biotechnol. , 99 : 4355-4360 2015 - Lévęque C, Ferracci G, Maulet Y, Mazuet C, Popoff MR, Blanchard MP, Seagar M, El Far O. An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E Scientific Reports, 5 : 17953 2015 - Hammond C, El Far O, Seagar M. Neurotransmitter release Cellular and molecular neurophysiology 2015 - El Far O, Seagar M. The synaptic vesicle V-ATPase: a regulatory link between loading and fusion? Presynaptic terminals 2015 - Lévęque C, Ferracci G, Maulet Y, Mazuet C, Popoff MR, Blanchard MP, Seagar M, El Far O. An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E. Scientific Reports, 5:17953 2014 - Lévęque C, Ferracci G, Maulet Y, Mazuet C, Popoff M, Seagar M, El Far O. Direct biosensor detection of botulinum neurotoxin endopeptidase activity in sera from patients with type A botulism Biosensors and Bioelectronics, 57 : 207-212 2014 - Mlayah-Bellalouna S, Dufour M, Mabrouk S, Mejdoub, H, Carlier E, Othman H, Belghazi M, Tarbe M, Goaillard JM, Gigmes D, Seagar M, El Ayeb M, Debanne D, Srairi-Abid N. AaTX1, from Androctonus australis scorpion venom: Purification, synthesis and characterization in dopaminergic neurons Toxicon, 92 : 14-23 2013 - Lévęque C, Ferracci G, Maulet Y, Grand-Masson C, Blanchard MP, Seagar M, El Far O. A substrate sensor chip to assay the enzymatic activity of Botulinum neurotoxin A. Biosensors & Bioelectronics, 49 : 276-281 2011 - El Far O, Seagar M. A role for V-ATPase subunits in synaptic vesicle fusion? Journal of Neurochemistry, 117 : 603-612 2011 - El Far O, Seagar M. SNARE, V-ATPase and neurotransmission Med Sci (Paris), 27 : 28-31 2011 - Ferracci G, Marconi S, Mazuet C, Jover E, Blanchard MP, Seagar M, Popoff M, Lévęque C. A label-free biosensor assay for botulinum neurotoxin B in food and human serum. Anal Biochem, 410 : 281-288 2010 - Di Giovanni J, Boudkkazi S, Mochida S, Bialowas A, Samari N, Lévęque C, Youssouf F, Brechet A, Iborra C Maulet Y, Moutot N, Debanne D, Seagar M, El Far O. V-ATPase membrane sector associates with synaptobrevin to modulate neurotransmitter release. Neuron, 67 : 268-279 2010 - Di Giovanni J, Iborra C, Maulet Y, Lévęque C, El Far O, Seagar M. Calcium-dependent regulation of SNARE-mediated membrane fusion by calmodulin J Biol Chem, 285 : 23665-23675 2010 - Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proc Natl Acad Sci USA, 107 : 3517-3521 2008 - Marconi S, Ferracci G, Berthomieu M, Kozaki S, Miquelis R, Boucraut J, Seagar M, Lévęque C. A protein chip membrane-capture assay for botulinum neurotoxin activity. Toxicol Appl Pharmacol, 233 : 439-446 2008 - Moutot N, De Haro L, Seagar M. Distinct evolution of calcium channel antibody types in Lambert-Eaton myasthenic syndrome. J Neuroimmunol, 197 : 47-53 2008 - Marqueze B, Moutot N, Sakkou-Norton M, Lévęque C, Ji Y, Cornet V, Hsiao WL, Seagar M. Toxicity and endocytosis of spinocerebellar ataxia type 6 polyglutamine domains: role of myosin IIb. Traffic, 9 : 1088-1100 2007 - Ji Y, Ferracci G, Warley A, Ward M, Leung KY, Samsuddin S, Lévęque C, Queen L, Reebye V, Pal P, Gkaliagkousi E, Seagar M, Ferro A. beta-Actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc Natl Acad Sci U S A, 104 : 8839-8844 2006 - Lacoste C, Barthaux V, Iborra C, Seagar M, Erard-Garcia M. MAU-8 is a Phosducin-like Protein required for G protein signaling in C. elegans. Dev Biol, 294 : 181-191 2006 - Moutot N, Mansuelle P, Alcaraz G, Dos Santos RG, Cordeiro MN, De Lima ME, Seagar M, Van Renterghem C. Phoneutria nigriventer toxin 1: a novel, state-dependent inhibitor of neuronal sodium channels that interacts with micro conotoxin binding sites. Mol Pharmacol, 69 : 1931-1937 2006 - Moutot N, De Haro L, Santos RG, Mori Y, Seagar M. Phoneutria nigriventer omega-Phonetoxin IIA: a new tool for anti-calcium channel autoantibody assays in Lambert-Eaton myasthenic syndrome. Neurobiol Dis, 22 : 57-63 2005 - Ferracci G, Miquelis R, Kozaki S, Seagar M, Lévęque C. Synaptic vesicle chips to assay botulinum neurotoxins. Biochem J, 391 : 659-66 2004 - Ferracci G, Seagar M, Joël C, Miquelis R, Lévęque C. Real time analysis of intact organelles using surface plasmon resonance. Anal Biochem, 334 : 367-375 2004 - Moutot N, De Haro L, Seagar M. [Dosage and specificity of anti-calcium channel antibodies in Lambert-Eaton myasthenic syndrome]. Rev Neurol (Paris), 165 : S28-S34 2004 - De Haro L, Ferracci G, Opi S, Iborra C, Quetglas S, Miquelis R, Lévęque C, Seagar M. Ca2+/calmodulin transfers the membrane-proximal lipid-binding domain of the v-SNARE synaptobrevin from cis to trans bilayers. Proc Natl Acad Sci U S A, 101 : 1578-1583 2003 - De Haro L, Quetglas S, Iborra C, Lévęque C, Seagar M. Calmodulin-dependent regulation of a lipid binding domain in the v-SNARE synaptobrevin and its role in vesicular fusion. Biol Cell, 95 : 459-464 2002 - Quetglas S, Iborra C, Sasakawa N, De Haro l, Kumakura K, Sato K, Lévęque C, Seagar M. Calmodulin and lipid binding to synaptobrevin regulates calcium-dependent exocytosis. EMBO J, 21 : 3970-3979 2002 - Dos Santos RG, Van Renterghem C, Moutot N, Mansuelle P, Cordeiro MN, Diniz CR, Mori Y, De Lima ME, Seagar M Phoneutria nigriventer omega-phonetoxin IIA blocks the Cav2 family of calcium channels and interacts with omega-conotoxin-binding sites. J Biol Chem, 277 : 13856-13862 2001 - Ashton AC, Volynski KE, Lelianova VG, Orlova EV, Van Renterghem C, Canepari M, Seagar M, Ushkaryov YA. alpha-Latrotoxin, acting via two Ca2+-dependent pathways, triggers exocytosis of two pools of synaptic vesicles. J Biol Chem, 276 : 44695-44703 2001 - Minami K, Raymond C, Moutot N, Ohtake A, Van Renterghem C, Takahashi M, Seagar MMori Y, Sato K. Role of Thr(11) in the binding of omega-conotoxin MVIIC to N-type Ca2+ channels. FEBS Lett, 491 : 127-130 2001 - Seagar M, Quetglas S, Iborra C, Lévęque C. Le complexe SNARE au coeur de la fusion membranaire Med. Sci, 5 : 669-674 2000 - Couraud F, Seagar M. [Calcium channels and migraine]. Pathol Biol (Paris), 48 : 642-647 2000 - Van Renterghem C, Iborra C, Moutot N, Lelianova V, Ushkaryov Y, Seagar M. alpha-latrotoxin forms calcium-permeable membrane pores via interactions with latrophilin or neurexin. Eur J Neurosci, 12 : 3953-3962 2000 - Quetglas S, Lévęque C, Miquelis R, Sato K, Seagar M. Ca2+-dependent regulation of synaptic SNARE complex assembly via a calmodulin- and phospholipid-binding domain of synaptobrevin. Proc Natl Acad Sci U S A, 97 : 9695-9700 2000 - Hermitte L, Moutot N, Boucraut J, Barone R, Atlan-Gepner C, Seagar M, Pouget J, Kleisbauer JP, Couraud F. Humoral immunity against glutamic acid decarboxylase and tyrosine phosphatase IA-2 in Lambert-Eaton myasthenic syndrome. J Clin Immunol, 20 : 287-293 2000 - Marqueze B, Berton F, Seagar M. Synaptotagmins in membrane traffic: which vesicles do the tagmins tag? Biochimie, 82 : 409-420 2000 - Berton F, Cornet V, Iborra C, Garrido J, Dargent B, Fukuda M, Seagar M, Marqueze B. Synaptotagmin I and IV define distinct populations of neuronal transport vesicles. Eur J Neurosci, 12 : 1294-1302 2000 - Sampo B, Tricaud N, Lévęque C, Seagar M, Couraud F, Dargent B. Direct interaction between synaptotagmin and the intracellular loop I-II of neuronal voltage-sensitive sodium channels. Proc Natl Acad Sci U S A, 97 : 3666-3671 2000 - Sato K, Raymond C, Moutot N, Sasaki T, Ohtake A, Minami K, Van Renterghem C, Kim JI, Takahashi M, Seagar M. Binding of Ala-scanning analogs of omega-conotoxin MVIIC to N- and P/Q-type calcium channels. FEBS Lett, 469 : 147-159 2000 - Sato K, Raymond C, Moutot NSasaki T, Ohtake A, Minami K, Van Renterghem C, Takahashi M, Seagar M. Binding of six chimeric analogs of omega-conotoxin MVIIA and MVIIC to N- and P/Q-type calcium channels. Biochem Biophys Res Commun, 269 : 254-256 2000 - Lévęque C, Boudier JA, Takahashi M, Seagar M. Calcium-dependent dissociation of synaptotagmin from synaptic SNARE complexes. J Neurochem, 74 : 367-374 1999 - Boudier JA, Moutot N, Boudier JL, Iborra C, Takahashi M, Seagar M. Redistribution of presynaptic proteins during alpha-latrotoxin-induced release of neurotransmitter and membrane retrieval at the frog neuromuscular junction. Eur J Neurosci, 11 : 3449-3456 1999 - Seagar M, Lévęque C, Charvin N, Marqueze B, Moutot N, Boudier JA, Boudier JL, Shoji-Kasai Y, Sato K. Interactions between proteins implicated in exocytosis and voltage-gated calcium channels. Philos Trans R Soc Lond B Biol Sci, 354 : 289-297 1999 - Raymond C, Walker D, Bichet D, Iborra C, Moutot N, Seagar M, De Waard M. Antibodies against the beta subunit of voltage-dependent calcium channels in Lambert-Eaton myasthenic syndrome. Neuroscience, 90 : 269-277 1998 - Seagar M, Takahashi M. Interactions between presynaptic calcium channels and proteins implicated in synaptic vesicle trafficking and exocytosis. J Bioenerg Biomembr, 30 : 347-356 1998 - Lévęque C, Pupier S, Marqueze B, Geslin L, Kataoka M, Takahashi M, De Waard M, Seagar M. Interaction of cysteine string proteins with the alpha1A subunit of the P/Q-type calcium channel. J Biol Chem, 273 : 13488-13492 1998 - Seagar M, Lévęque C, Charvin N, Marqueze B, Moutot N, Boudier JA, Boudier JL, Kataoka M, Shoji-Kaisai Y, Takahashi M. Interactions between proteins implicated in exocytosis and voltage-gated calcium channels. Eur J Neurosci, 10 : 12501 1998 - Seagar M, Moutot N, Raymond C, Charvin N, Lévęque C, Walker D, De Waard M. Omega-conotoxins : diagnostic tools for an autoimmune disease of the neuromuscular juction Toxicon, 36 : 1730-1731 1997 - Sato K, Raymond C, Moutot N, Sasaki T, Omori A, Ohtaki A, Kim JI, Kohno T, Takahashi M, Seagar M. Binding of chimeric analogs of omega-conotoxin MVIIA and MVIIC to the N- and P/Q-type calcium channels. FEBS Lett, 414 : 480-484 1997 - Charvin N, Lévęque C, Walker D, Berton F, Raymond C, Kataoka M, Shoji-Kasai Y, Takahashi M, De Waard M, Seagar M. Direct interaction of the calcium sensor protein synaptotagmin I with a cytoplasmic domain of the alpha1A subunit of the P/Q-type calcium channel. EMO J, 16 : 4591-4596 1997 - Pupier S, Lévęque C, Marqueze B, Kataoka M, Takahashi M, Seagar M. Cysteine string proteins associated with secretory granules of the rat neurohypophysis. J Neurosci, 17 : 2722-2727 1997 - Berton F, Iborra C, Boudier JA, Seagar M, Marqueze B. Developmental regulation of synaptotagmin I, II, III, and IV mRNAs in the rat CNS. J Neurosci, 17 : 1206-1216 1996 - Arsac C, Raymond C, Moutot N, Dargent B, Couraud F, Poget J, Seagar M. Immunoassays fail to detect antibodies against neuronal calcium channels in amyotrophic lateral sclerosis serum. Ann Neurol, 40 : 695-700 1996 - Moutot N, Charvin N, Lévęque C, Sato K, Nishiki T, Kozaki S, Takahashi M, Seagar M. Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. J Biol Chem, 271 : 6567-6570 1996 - Boudier JA, Charvin N, Boudier JL, Fathallah M, Tagaya M, Takahashi M, Seagar M. Distribution of components of the SNARE complex in relation to transmitter release sites at the frog neuromuscular junction. Eur J Neurosci, 8 : 545-552 1995 - El Far O, Marqueze B, Lévęque C, Moutot N, Lang B, Newsom-Davis J, Yoshida A, Takahashi M, Seagar M Antigens associated with N- and L-type calcium channels in Lambert-Eaton myasthenic syndrome. J Neurochem, 64 : 1696-1702 1995 - El Far O, Charvin N, Lévęque C, Moutot N, Takahashi M, Seagar M. Interaction of a synaptobrevin (VAMP)-syntaxin complex with presynaptic calcium channels. FEBS Lett, 361 : 101-105 1995 - Kim JI, Takaashi M, Moutot N, Seagar M, Ohtake A, Sato K. Tyr13 is essential for the binding of omega-conotoxin MVIIC to the P/Q-type calcium channel. Biochem Biophys Res Commun, 214 : 305-309 1995 - Marqueze B, Boudier JA, Mizuta M, Inagaki M, Seino S, Seagar M. Cellular localization of synaptotagmin I, II, and III mRNAs in the central nervous system and pituitary and adrenal glands of the rat. J Neurosci, 15 : 4906-4917 1995 - Moutot N, Lévęque C, Sato K, Kato R, Takahashi M, Seagar M. Properties of omega conotoxin MVIIC receptors associated with alpha 1A calcium channel subunits in rat brain. FEBS Lett, 366 : 21-25 1995 - Angaut-Petit D, Juzans P, Molgo J, Faille L, Seagar M, Takahashi M, Shoji-Kasai Y. Mouse motor nerve terminal immunoreactivity to synaptotagmin II during sustained quantal transmitter release. Brain Res, 68 : 213-217 1995 - Moutot N, Lang B, Newsom-Davis J, Seagar M. Binding of Lambert-Eaton myasthenic syndrome IgG to synaptosomal proteins does not correlate with an inhibition of calcium uptake. Neurosci Lett, 187 : 11(-118 1995 - Seagar M, Moutot N, Lévęque C, Marqueze B, Pouget J. Anti-calcium channel autoantibodies in amyotrophic lateral sclerosis. A review of the evidence. Adv Neurol, 68 : 67-72 1994 - Lévęque C, El Far O, Moutot N, Sato K, Kato R, Takahashi M, Seagar M. Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. A complex implicated in synaptic vesicle exocytosis. J Biol Chem, 269 : 6306-6312 1993 - El Far O, Moutot N, Lévęque C, David P, Marqueze B, Lang B, Newom-Davis J, Hoshino T, Takahashi M, Seagar M. Synaptotagmin associates with presynaptic calcium channels and is a Lambert-Eaton myasthenic syndrome antigen. Ann N Y Acad Sci, 707 : 382-385 1993 - David P, Moutot N, Lévęque C, El Far O, Takahashi M, Seagar M. Interaction of synaptotagmin with voltage gated calcium channels: a role in Lambert-Eaton myasthenic syndrome? Neuromuscul Disord, 3 : 451-454 1993 - David P, El Far O, Moutot N, Poupon MF, Takahashi M, Seagar M. Expression of synaptotagmin and syntaxin associated with N-type calcium channels in small cell lung cancer. FEBS Lett, 326 : 135-139 1993 - Seagar M, Moutot N, Lévęque C, El Far O, David P, Marqueze B, Lang B, Newsom-Davis J, Hoshino T, Takahashi M. A Lambert-Eaton myasthenic syndrome antigen associated with presynaptic calcium channels. Biochem Soc Trans, 21 : 423-428 1993 - Moutot N, El Far O, Lévęque C, David P, Marqueze B, Lang B, Newsom-Davis J, Hoshino T, Takahashi M, Seagar M. Synaptotagmin: a Lambert-Eaton myasthenic syndrome antigen that associates with presynaptic calcium channels. J Physiol Paris, 87 : 37-41 1993 - McEnery MW, Snowman AM, Seagar M, Copeland TD, Takahashi M. Immunological characterization of proteins associated with the purified omega-conotoxin GVIA receptor. Ann N Y Acad Sci, 707 : 386-391 1993 - Lang B, Johnston I, Leys K, Elrington G, Marqueze B, Lévęque C, Moutot N, Seagar M, Hoshino T, Takahashi M, Sugimori M, Cherksey BD, Llinas R. Autoantibody specificities in Lambert-Eaton myasthenic syndrome. Ann N Y Acad Sci, 681 : 382-393 1993 - Grignon S, Seagar M, Couraud F. Omega-conotoxin sensitive calcium channels in cerebellar granule cells are not coupled to [3H]glutamate release. Neurosci Lett, 155 : 8č-91 1992 - Lévęque C, Hoshino T, David P, Shoji-Kasai Y, Leys K, Omori A, Lang B , El Far O, Sato K, Newsom-Davis J, Takahashi M, Moutot N, Seagar M. The synaptic vesicle protein synaptotagmin associates with calcium channels and is a putative Lambert-Eaton myasthenic syndrome antigen. Proc Natl Acad Sci U S A, 89 : 3625-3629 1992 - De Waard M, Seagar M, Feltz A, Couraud F. Inositol phosphate regulation of voltage-dependent calcium channels in cerebellar granule neurons. Neuron, 9 : 497-503 1990 - Lai Y, Seagar M, Takahashi M, Catterall WA. Cyclic AMP-dependent phosphorylation of two size forms of alpha 1 subunits of L-type calcium channels in rat skeletal muscle cells. J Biol Chem, 265 : 20839-20948 1990 - Lévęque C, Marqueze B, Couraud F, Seagar M. Polypeptide components of the apamin receptor associated with a calcium activated potassium channel. FEBS Lett, 275 : 185-189 1990 - Moutot N, Seagar M, Couraud F. Subtypes of voltage-sensitive calcium channels in cultured rat brain neurons. Neurosci Lett, 115 : 300-306 1987 - Seagar M, Deprez P, Moutot N, Couraud. Detection and photoaffinity labeling of the Ca2+-activated K+ channel-associated apamin receptor in cultured astrocytes from rat brain. Brain Res, 411 : 226-230